Cell Line Characteristics

Repository Number:

AG05965

Subcollection:

Specially Characterized Fibroblasts

Sample Description:

MRC-5 - Normal Human Fetal Lung Fibroblast

Cell Type:

Fibroblast

Cell Subtype:

Lung

Genus species:

Homo sapiens

Common Name:

human

Clinically Affected:

No

Age:

14 FW

Gender:

Male

Race:

Caucasian

Relation to Proband:

proband

Remarks:

The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B.

ISCN:

46,XY

Price:

$75

Culture Protocol

Split Ratio:

1:5

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

Not determined

Population Doubling at Freeze:

27

In vitro Lifespan:

Not determined

Split Ratio:

1:5

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

Not determined

Population Doubling at Freeze:

40

In vitro Lifespan:

Not determined

Split Ratio:

1:12

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

Not determined

Population Doubling at Freeze:

17

In vitro Lifespan:

Not determined

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN:

Species of Origin Confirmed by Chromosome Analysis

IDENTIFICATION OF SPECIES OF ORIGIN:

Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis

Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, J Biol Chem 270:6243-5 (1995) The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH.
PubMed ID: 7534307

Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Cancer Res 54:1791-4 (1994) Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line.
PubMed ID: 7511052

Kilfeather SA, Collins D, McCormack P, Cotter T, O'Malley K, Mech Ageing Dev 63:247-56 (1992) The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response.
PubMed ID: 1319530

Jacobs, Nature 277:168 (1970), Characteristics of a human diploid cell designated MRC-5.