WI-38 Aborted Fetal Cell Line
Coriell Cell Repositories http://locus.umdnj.edu
Related Information on MRC-5 Aborted Fetal Cell Line
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Cell
Line Characteristics |
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AG06814 |
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Specially
Characterized Fibroblasts |
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Sample
Description: |
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DNA
Sample: |
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Fibroblast |
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Lung |
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Genus
species: |
Homo
sapiens |
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Common
Name: |
human |
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Clinically
Affected: |
No |
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12
FW |
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Female |
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Caucasian |
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proband |
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The
WI-38 cell line was developed in July 1962 from lung tissue taken from a
therapeutically aborted fetus of about 3 months gestational age. Cells
released by trypsin digestion of the lung tissue were used for the primary
culture. The cell morphology is fibroblast-like. The karyotype is 46,XX;
normal diploid female. A maximum lifespan of 50 population doublings for
this culture was obtained at the Repository. A thymidine labelling index
of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture
of WI-38 is an expansion from passage 9 frozen cells obtained from the
submitter. |
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46,XX |
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Price: |
$75 |
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Culture
Protocol |
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1:5 |
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37
C |
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5% |
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MEM
Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM
L-glutamine |
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15%
fetal bovine serum Uninactivated |
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|
None
specified |
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trypsin-EDTA |
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Frozen
at Passage: |
22 |
|
22 |
|
|
Not
determined |
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1:4 |
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37
C |
|
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5% |
|
|
MEM
Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM
L-glutamine |
|
|
15%
fetal bovine serum Uninactivated |
|
|
None
specified |
|
|
trypsin-EDTA |
|
|
Frozen
at Passage: |
38 |
|
44 |
|
|
Not
determined |
|
|
1:5 |
|
|
37
C |
|
|
5% |
|
|
MEM
Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM
L-glutamine |
|
|
15%
fetal bovine serum Uninactivated |
|
|
None
specified |
|
|
trypsin-EDTA |
|
|
Frozen
at Passage: |
26 |
|
34 |
|
|
Not
determined |
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|
Characterizations |
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IDENTIFICATION
OF SPECIES OF ORIGIN: |
Species
of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate
Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by
Chromosome Analysis |
References
|
Borsi
L, Balza E, Gaggero B, Allemanni G, Zardi L, J Biol Chem 270:6243-5 (1995) The
alternative splicing pattern of the tenascin-C pre-mRNA is controlled by
the extracellular pH. |
|
Venable
ME, Lee JY, Smyth MJ, Bielawska A, Obeid LM, J Biol Chem 270:30701-8 (1995) Role of
ceramide in cellular senescence. |
|
Dimri
GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens
M, Rubelj I, Pereira-Smith O, et al, Proc Natl Acad Sci U S A 92:9363-7 (1995) A
biomarker that identifies senescent human cells in culture and in aging
skin in vivo. |
|
Dimri
GP, Campisi J,
Exp Cell Res 212:132-40 (1994) Altered profile of transcription
factor-binding activities in senescent human fibroblasts. |
|
Dimri
GP, Hara E, Campisi J, J Biol Chem 269:16180-6 (1994) Regulation of two E2F-related
genes in presenescent and senescent human fibroblasts. |
|
Venable
ME, Blobe GC, Obeid LM, J Biol Chem 269:26040-4 (1994) Identification of a defect in
the phospholipase D/diacylglycerol pathway in cellular senescence. |
|
Kunisada
T, Miller CD, Schneider EL, Mutat Res 237:75-81 (1990) Ultraviolet light-induced DNA
damage in transcribed sequences: no change in repair with age. |
|
Ganguly
T, Duker NJ, Mutat
Res 235:137-46 (1990) Differential cell cycle modulation of human DNA
glycosylases against oxidized pyrimidines. |
|
Pereira-Smith
OM, Stein GH, Robetorye S, Meyer-Demarest S, J Cell Physiol 143:222-5 (1990) Immortal
phenotype of the HeLa variant D98 is recessive in hybrids formed with
normal human fibroblasts. |
|
Giordano
T, Kleinsek D, Foster DN, Exp Gerontol 24:501-13 (1989) Increase in abundance of a
transcript hybridizing to elongation factor I alpha during cellular
senescence and quiescence. |
|
McNearney
T, Ballard L, Seya T, Atkinson JP, J Clin Invest 84:538-45 (1989) Membrane cofactor
protein of complement is present on human fibroblast, epithelial, and
endothelial cells. |
|
Giordano
T, Foster DN,
Exp Cell Res 185:399-406 (1989) Identification of a highly abundant
cDNA isolated from senescent WI-38 cells. |
|
Gibson
JM, Milam SB, Klebe RJ, Mech Ageing Dev 48:101-10 (1989) Late passage cells display
an increase in contractile behavior. |
|
Kondo
H, Kasuga H, Noumura T, Exp Cell Res 158:342-8 (1985) The heterogeneity of human
fibroblasts as determined from the effects of hydrocortisone on cell
growth and specific dexamethasone binding. |
|
Hayflick,
Exp Cell Res 37:614 (1965), The limited in vitro lifetime of human
diploid cell strains. |
|
Hayflick,
Exp Cell Res 25:585 (1961), The serial cultivation of human diploid
cell strains. |