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Cell Line Characteristics

Repository Number:

AG06814

Subcollection:

Specially Characterized Fibroblasts

Sample Description:

WI-38 - Normal Human Fetal Lung Fibroblast

DNA Sample:

NA06814

Cell Type:

Fibroblast

Cell Subtype:

Lung

Genus species:

Homo sapiens

Common Name:

human

Clinically Affected:

No

Age:

12 FW

Gender:

Female

Race:

Caucasian

Family Number:

1053

Relation to Proband:

proband

Remarks:

The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter.

ISCN:

46,XX

Price:

$75

Culture Protocol

Split Ratio:

1:5

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

22

Population Doubling at Freeze:

22

In vitro Lifespan:

Not determined

Split Ratio:

1:4

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

38

Population Doubling at Freeze:

44

In vitro Lifespan:

Not determined

Split Ratio:

1:5

Temperature:

37 C

Percent CO2:

5%

Medium:

MEM Eagle-Earle BSS 2X conc. of essen. & non-essen AA & Vit. with 2mM L-glutamine

Serum:

15% fetal bovine serum Uninactivated

Substrate:

None specified

Subcultivation Method:

trypsin-EDTA

Frozen at Passage:

26

Population Doubling at Freeze:

34

In vitro Lifespan:

Not determined

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN:

Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis

Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, J Biol Chem 270:6243-5 (1995) The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH.
PubMed ID: 7534307

Venable ME, Lee JY, Smyth MJ, Bielawska A, Obeid LM, J Biol Chem 270:30701-8 (1995) Role of ceramide in cellular senescence.
PubMed ID: 8530509

Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, et al, Proc Natl Acad Sci U S A 92:9363-7 (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
PubMed ID: 7568133

Dimri GP, Campisi J, Exp Cell Res 212:132-40 (1994) Altered profile of transcription factor-binding activities in senescent human fibroblasts.
PubMed ID: 8174635

Dimri GP, Hara E, Campisi J, J Biol Chem 269:16180-6 (1994) Regulation of two E2F-related genes in presenescent and senescent human fibroblasts.
PubMed ID: 8206919

Venable ME, Blobe GC, Obeid LM, J Biol Chem 269:26040-4 (1994) Identification of a defect in the phospholipase D/diacylglycerol pathway in cellular senescence.
PubMed ID: 7929315

Kunisada T, Miller CD, Schneider EL, Mutat Res 237:75-81 (1990) Ultraviolet light-induced DNA damage in transcribed sequences: no change in repair with age.
PubMed ID: 2164149

Ganguly T, Duker NJ, Mutat Res 235:137-46 (1990) Differential cell cycle modulation of human DNA glycosylases against oxidized pyrimidines.
PubMed ID: 2308590

Pereira-Smith OM, Stein GH, Robetorye S, Meyer-Demarest S, J Cell Physiol 143:222-5 (1990) Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts.
PubMed ID: 2332448

Giordano T, Kleinsek D, Foster DN, Exp Gerontol 24:501-13 (1989) Increase in abundance of a transcript hybridizing to elongation factor I alpha during cellular senescence and quiescence.
PubMed ID: 2483690

McNearney T, Ballard L, Seya T, Atkinson JP, J Clin Invest 84:538-45 (1989) Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells.
PubMed ID: 2474570

Giordano T, Foster DN, Exp Cell Res 185:399-406 (1989) Identification of a highly abundant cDNA isolated from senescent WI-38 cells.
PubMed ID: 2480908

Gibson JM, Milam SB, Klebe RJ, Mech Ageing Dev 48:101-10 (1989) Late passage cells display an increase in contractile behavior.
PubMed ID: 2739467

Kondo H, Kasuga H, Noumura T, Exp Cell Res 158:342-8 (1985) The heterogeneity of human fibroblasts as determined from the effects of hydrocortisone on cell growth and specific dexamethasone binding.
PubMed ID: 4007059

Hayflick, Exp Cell Res 37:614 (1965), The limited in vitro lifetime of human diploid cell strains.

Hayflick, Exp Cell Res 25:585 (1961), The serial cultivation of human diploid cell strains.